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Literature summary extracted from

  • Kristensen, C.; Morant, M.; Olsen, C.E.; Ekstrom, C.T.; Galbraith, D.W.; Moller, B.L.; Bak, S.
    Metabolic engineering of dhurrin in transgenic Arabidopsis plants with marginal inadvertent effects on the metabolome and transcriptome (2005), Proc. Natl. Acad. Sci. USA, 102, 1779-1784.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.14.36 agriculture transgenic Arabidopsis thaliana plants expressing CYP79A1, CYP71E1, and UGT85B1 from Sorghum bicolor, i.e. the entire biosynthetic pathway for the tyrosine-derived cyanogenic glucoside dhurrin, accumulate 4% dry-weight dhurrin with marginal inadvertent effects on plant morphology, free amino acid pools, transcriptome, and metabolome. Plants expressing only CYP79A1 accumulate 3% dry weight of the tyrosine-derived glucosinolate, 4-hydroxybenzylglucosinolate with no morphological pleitropic effects. Insertion of CYP79A1 plus CYP71E1 results in stunted plants, transcriptome alterations, accumulation of numerous glucosides derived from detoxification of intermediates in the dhurrin pathway, and in loss of the brassicaceae-specific UV protectants sinapoyl glucose and sinapoyl malate and kaempferol glucosides. The accumulation of glucosides in the plants expressing CYP79A1 and CYP71E1 is not accompanied by induction of glycosyltransferases Sorghum bicolor
1.14.14.37 agriculture transgenic Arabidopsis thaliana plants expressing CYP79A1, CYP71E1, and UGT85B1 from Sorghum bicolor, i.e. the entire biosynthetic pathway for the tyrosine-derived cyanogenic glucoside dhurrin, accumulate 4% dry-weight dhurrin with marginal inadvertent effects on plant morphology, free amino acid pools, transcriptome, and metabolome. Plants expressing only CYP79A1 accumulate 3% dry weight of the tyrosine-derived glucosinolate, 4-hydroxybenzylglucosinolate with no morphological pleitropic effects. Insertion of CYP79A1 plus CYP71E1 results in stunted plants, transcriptome alterations, accumulation of numerous glucosides derived from detoxification of intermediates in the dhurrin pathway, and in loss of the brassicaceae-specific UV protectants sinapoyl glucose and sinapoyl malate and kaempferol glucosides. The accumulation of glucosides in the plants expressing CYP79A1 and CYP71E1 is not accompanied by induction of glycosyltransferases Sorghum bicolor
2.4.1.85 agriculture it is possible to engineer plants (Arabidopsis thaliana) which express the high flux pathway for dhurrin synthesis Sorghum bicolor

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.85 transgenic Arabidopsis thaliana plants expressing the entire biosynthetic pathway for the tyrosine-derived cyanogenic glucoside dhurrin as accomplished by insertion of CYP79A1, CYP71E2, and UFT85B1 are shown to accumulate 4% dry-weight dhurrin with marginal inadvertent effects on plant morphology, free amino acid pools, transcriptome, and metabolome Sorghum bicolor

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.36 Sorghum bicolor Q43135
-
-
1.14.14.37 Sorghum bicolor O48958
-
-
2.4.1.85 Sorghum bicolor
-
-
-

Synonyms

EC Number Synonyms Comment Organism
1.14.14.36 CYP79A1
-
 Sorghum bicolor
1.14.14.37 CYP71E1
-
 Sorghum bicolor
2.4.1.85 UGT85B1
-
 Sorghum bicolor